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2010 Jul-Sep; Vol 1, No 3:e3 ____________________________________________________________________________
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Protein Adsorption to Surface Chemistry and Crystal Structure Modification of Titanium Surfaces
Ryo Jimbo1,2, Mikael Ivarsson3, Anita Koskela3, Young-Taeg Sul2,4, Carina B. Johansson5
1Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden 2Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden 3Clinical Research Center, Örebro University Hosptial, Örebro, Sweden 4Institute for Clinical Dental Research, Korea University, Seoul, South Korea 5Department of Clinical Medicine, School of Health and Medical Sciences, Örebro University, Örebro, Sweden
Corresponding author: Ryo Jimbo Department of Prosthodontics, Faculty of Odontology, Malmö University 205 06 Malmö Sweden Phone: +46 40 6658 502 Fax: +46 40 6658 503 E-mail: ryo.jimbo@mah.se
Copyright © Jimbo R, Ivarsson M, Koskela A, Sul YT, Johansson CB. Accepted for publication in the JOURNAL OF ORAL & MAXILLOFACIAL RESEARCH (http://www.ejomr.org), 18 May 2010.
This is an open-access article, first published in the JOURNAL OF ORAL & MAXILLOFACIAL RESEARCH, distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work and is properly cited. The copyright, license information and link to the original publication on (http://www.ejomr.org) must be included.
ABSTRACT
Objectives: To observe the early adsorption of extracellular matrix and blood plasma proteins to magnesium-incorporated titanium oxide surfaces, which has shown superior bone response in animal models. Material and Methods: Commercially pure titanium discs were blasted with titanium dioxide (TiO2) particles (control), and for the test group, TiO2 blasted discs were further processed with a micro-arc oxidation method (test). Surface morphology was investigated by scanning electron microscopy, surface topography by optic interferometry, characterization by X-ray photoelectron spectroscopy (XPS), and by X-ray diffraction (XRD) analysis. The adsorption of 3 different proteins (fibronectin, albumin, and collagen type I) was investigated by an immunoblotting technique. Results: The test surface showed a porous structure, whereas the control surface showed a typical TiO2 blasted structure. XPS data revealed magnesium-incorporation to the anodic oxide film of the surface. There was no difference in surface roughness between the control and test surfaces. For the protein adsorption test, the amount of albumin was significantly higher on the control surface whereas the amount of fibronectin was significantly higher on the test surface. Although there was no significant difference, the test surface had a tendency to adsorb more collagen type I. Conclusions: The magnesium-incorporated anodized surface showed significantly higher fibronectin adsorption and lower albumin adsorption than the blasted surface. These results may be one of the reasons for the excellent bone response previously observed in animal studies.
J Oral Maxillofac Res 2010 (Jul-Sep);1(3):e3 doi: 10.5037/jomr.2010.1303 Accepted for publication: 18 May 2010
Keywords: titanium dioxide; magnesium; immunoblotting; fibronectins; albumins; collagen type I.
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