Purified albumin, fibronectin, or collagen type I were allowed to attach to surfaces for 16 h at 37 ˚C.
Relative binding was measured by electrophoresis followed by Coomassie blue staining or immunoblotting of solubilized proteins (A).
Significant differences by densitometry (B - D) using paired Student's t-test (P ≤ 0.05) are indicated (*).