Figure 2

Figure 2.

A = A microradiograph of a developing human molar tooth with an advanced carious lesion. Undecalcified frozen section (10 micrometer thick) of an extracted human molar tooth. Original magnification x8.
B = Localization of alkaline phosphatase activity in the odontoblasts, predentine, cementum, in remaining parts of the periodontal ligament, and some pulpal cells. Substrate: 1-naphthyl acid phosphate, Fast Blue RR, pH 9.2). Original magnification x6.
C = Higher magnification of the localization of alkaline phosphatase activity in pulpal cells under the advanced carious lesion with pulp perforation and pulpal inflammation. Note the disappearance of enzyme activity in odontoblasts in the site of carious exposure and appearance in dentinal tubules in soft carious dentin. Original magnification x48.
D = Higher magnification of the same area of pulp horn showing the presence of arylaminopeptidase activity in the fibroblasts/progenitor/stem cells or inflammatory cells in the inflamed pulp horn. Substrate: N-L-arginyl-2-naphthylamine, Fast Blue B, pH, 6.8. Original magnification x48.

The picture is originally published in The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology, 296, p. 565, 2013, and the reproduction granted by WILEY/ the Copyright Clearence Center Inc, License Number 3213520064351.