Study | Type of study | Model investigated | Patient no. | Cell source | Mobilization procedure (+\-) and material | Assay of mobilization |
Apheresis procedure (+\-) and method |
Osteogenic differentiation potential | Markers found |
---|---|---|---|---|---|---|---|---|---|
Smiler et al. [4] | Research article | Humans | 6 | BM PB, | - | - | +, Modern flow cytometer | - | CD105+ Using 6-color flow cytometry |
Castillo-Cardiel et al. [5] | Randomized clinical trial | Humans | 20 | Adipose tissue | - | - | - | +, using panoramic radiograph and CT | - |
McNulty et al. [6] | Pilot Study | Mice | 97 | PB | +, AMD3100, bone marrow ablation | CFU, flow cytometry, micro-CT. | - | - | - |
Dhar et al. [7] | Clinical trial | Equine | 10 | PB, Adipose tissue | +, hyperbaric oxygen therapy | Markers expression, cytospin. | Ficoll.+ | + alizarin red staining solution | CD51, CD90, CD105 |
Marycz et al. [8] | Research article | Mice | 90 | BM and PB | +, endurance exercise | Clonogenic assay, ELISA | - | - | - |
Humans | 12 | BM and PB | ELISA | - | - | - | |||
Lyahyai et al. [9] | Research article | Sheep-Ovine | 6 | PB | - | - | +, NA | + alizarin red staining solution (cytochemical) | CD105, CD73, CD90 |
Kassis et al. [10] | Original article | Human | NA | PB | +, G-CSF | NA | +, COBE Spectra apheresis system + isolation by FMB. | + nitro-blue tetrazolium/indolylp-hosphate staining to observe alkaline phosphatase activity of the osteogenic cells | CD45, CD105, CD90, vimentin, fibronectin. |
Li et al. [11] | Original research | Mice | NA | PB | - | - | - | + alizarin red staining solution | CD45-(BD-MSC) using coculture system from PB |
Sato et al. [12] | Research article | Feline | 22 | PB | - | - | + | + alizarin red staining solution (cytochemical) | CD44, CD90 |
Motlló et al. [33] | Research article | Human | 133 | PB | +, G-CSF | EPICS XL-MCL flow cytometer. | +, COBE Spectra apheresis system | - | - |
Anguita-Compagnon et al. [34] | Retrospective study | Humans | 31 donors | PB | +, G-CSF | Flow cytometry, clonogenic assay. | + COBE Spectra cell processor | - | - |
NA = not applicable; PB = peripheral blood; BM = bone marrow; CT = computed tomography. |