Antibody Source Clone Pretreatment Dilution Incubation
(min)
Alpha smooth muscle actin Dako, Glostrup, Denmark 1A4 No treatment 1:100 30
Vimentin Dako, Glostrup, Denmark V9 Microwave
Citrate buffer 0.01M
pH 7.2 ,95°C, 15 min		 1:100 30
Desmin BioCare, Carmino Diablo,
CA, USA		 D33 Microwave
Citrate buffer 0.01M
pH 7.2, 95°C, 15 min		 1:100 30
Transforming
growth factor-beta1		 Spinge Biosense, Pleasaton,
CA, USA		 Polyclonal Microwave
Citrate buffer 0.01M
pH 6, 95°C, 10 min		 1:25 30