High magnification SEM images: A, B and C = formation of globular mineralized structures closely associated with the cell layer.
D = representative X-ray spectra of the mineralized deposits, showing the presence of Ca and P peaks.
E = levels of ionized calcium (Ca_i) in the culture medium throughout the 30-day culture time, showing the consumption of Ca_i from the medium due to the formation of calcium containing deposits in the cell layer.
^aSignificantly different from Implant I.