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NR4A3 (NOR-1) Immunostaining Shows Better Performance than DOG1 Immunostaining in Acinic Cell Carcinoma of Salivary Gland: a Preliminary Study J Oral Maxillofac Res 2021;12(1):e4 doi:10.5037/jomr.2021.12104 Abstract | HTML | PDF | XML |
NR4A3 (NOR-1) Immunostaining Shows Better Performance than DOG1 Immunostaining in Acinic Cell Carcinoma of Salivary Gland: a Preliminary Study
1Missouri School of Dentistry, A.T. Still University, Kirksville, Missouri, United States.
2Joint Base San Antonio-Lackland Air Force Base, San Antonio, Texas, United States.
3Department of Oral and Maxillofacial Surgery and Oral Pathology, Faculty of Dentistry, Obafemi Awolowo University, Ile-Ife, Nigeria.
4Department of Morbid Anatomy and Forensic Medicine, Obafemi Awolowo University Teaching Hospital, Ile-Ife, Nigeria.
4Department of Diagnostic Sciences, School of Dental Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, United States.
Corresponding Author:
Missouri School of Dentistry and Oral Health
A. T. Still University
800 W Jefferson Street, Kirksville, Missouri 63501
United States
Phone: +1 660 626 2843
E-mail: adepitanowosho@atsu.edu
ABSTRACT
Objectives: Acinic cell carcinoma of salivary gland harbours recurrent and specific chromosomal rearrangement [t(4;9)(q13;q31)], resulting in the translocation of secretory calcium-binding phosphoprotein gene cluster at 4q13 to nuclear receptor subfamily 4 group a member 3 at 9q31. This upregulates the transcription factor nuclear receptor subfamily 4 group A member 3, which can be detected by immunohistochemistry. The purpose of this pilot study is to evaluate the performance of nuclear receptor subfamily 4 group A member 3 immunostaining on whole-slide acinic cell carcinoma tissue, in comparison with discovered on GIST-1 immunostaining.
Material and Methods: We retrieved 6 cases of acinic cell carcinoma (AciCC), including 5 conventional low-grade and 1 dedifferentiated high-grade. Immunohistochemistry (IHC) for nuclear receptor subfamily 4 group A member 3 (NR4A3) and discovered on GIST-1 (DOG1) were performed at the University of Pittsburgh Medical Centre in Pittsburgh, Pennsylvania on all retrieved cases.
Results: The result shows that NR4A3 IHC shows better performance than DOG1 IHC: 5 of the 6 (83.3%) AciCC cases (including the dedifferentiated high-grade) demonstrated strong diffuse nuclear staining for NR4A3, also five AciCC cases (including the dedifferentiated high-grade) demonstrated weak to moderate membranous staining with variable distribution for DOG1. Moreover, only 3 (50%) cases showed complete membranous staining with DOG1.
Conclusions: This pilot study showed that nuclear receptor subfamily 4 group A member 3 immunostaining is a sensitive marker for acinic cell carcinoma and of better utility than discovered on GIST-1 immunostaining in making a diagnosis of acinic cell carcinoma.
J Oral Maxillofac Res 2021;12(1):e4
doi: 10.5037/jomr.2021.12104
Accepted for publication: 24 February 2021
Keywords: gene rearrangement; immunohistocytochemistry; malignant neoplasms.
To cite this article: NR4A3 (NOR-1) Immunostaining Shows Better Performance than DOG1 Immunostaining in Acinic Cell Carcinoma of Salivary Gland: a Preliminary Study J Oral Maxillofac Res 2021;12(1):e4 URL: http://www.ejomr.org/JOMR/archives/2021/1/e4/v12n1e4ht.htm |
Received: 11 December 2020 | Accepted: 24 February 2021 | Published: 31 March 2021
Copyright: © The Author(s). Published by JOMR under CC BY-NC-ND 3.0 licence, 2021.