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Immunohistochemical Study of Laminin-332 γ2 Chain and MMP-9 in High Risk of Malignant Transformation Oral Lesions and OSCC J Oral Maxillofac Res 2018;9(1):e3 doi:10.5037/jomr.2018.9103 Abstract | HTML | PDF | XML |
Immunohistochemical Study of Laminin-332 γ2 Chain and MMP-9 in High Risk of Malignant Transformation Oral Lesions and OSCC
1Dental Clinic, Federal University of Espírito Santo, Health of Sciences Center, Brazil.
2Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, Brazil.
3Department of Morphology, Health of Sciences Center, Federal University of Espírito Santo, Brazil.
Corresponding Author:
Department of Morphology, Health of Sciences Center
Federal University of Espírito Santo
Marechal Campos av., 1468, Maruípe, 29.040-090, Vitória, ES
Brazil
Phone: +552733357358
E-mail: leticia.souza@ufes.br
ABSTRACT
Objectives: Oral squamous cell carcinoma is associated with alterations in basement membrane. Laminin-332 is present in basal lamina and performs multiple biologic effects by γ2 chain. Matrix metalloproteinase acts disrupting extracellular components and was related to poor prognosis in cancer. Here, molecular profile of laminin-332 γ2 chain and matrix metalloproteinase-9 was assessed in oral lesions.
Material and Methods: The expression of laminin-332 γ2 chain and matrix metalloproteinase-9 (MMP-9) was examined by immunohistochemistry in 10 patients with high risk of malignant transformation oral lesions and 26 cases of oral squamous cell carcinoma (OSCC). Associations between microscopic and clinicopathologic features were established.
Results: Immunostaining of laminin-332 γ2 chain in high risk oral lesions was most detected in basement membrane which is continuous, while the majority of OSCC cases showed a discontinuous membrane (P = 0.001). It was observed a positive reaction for γ2 chain in invasive fronts and a higher expression in epithelial compartment of smoking patients with OSCC (P < 0.0001). In epithelium, MMP-9 expression was presented in all layers with no difference between lesions. However, an elevated immunostaining in stromal cells was associated with male patients (P = 0.0054), older than 60 years (P = 0.0101) and with OSCC.
Conclusions: Present study results support the hypothesis of changes in molecules expression in high risk oral lesions and oral squamous cell carcinoma. A relation between clinical and molecule profile was observed. Those molecules may represent a useful tool to predict oral cancer behaviour.
J Oral Maxillofac Res 2018;9(1):e3
doi: 10.5037/jomr.2018.9103
Accepted for publication: 10 March 2018
Keywords: carcinogenesis; laminin; matrix metalloproteinase 9; oral cancer; oral pathology; squamous cell carcinoma.
To cite this article: Immunohistochemical Study of Laminin-332 γ2 Chain and MMP-9 in High Risk of Malignant Transformation Oral Lesions and OSCC J Oral Maxillofac Res 2018;9(1):e3 URL: http://www.ejomr.org/JOMR/archives/2018/1/e3/v9n1e3.htm |
Received: 21 September 2017 | Accepted: 10 March 2018 | Published: 31 March 2018
Copyright: © The Author(s). Published by JOMR under CC BY-NC-ND 3.0 licence, 2018.