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Early Effects of P-15 on Human Bone Marrow Stem Cells J Oral Maxillofac Res 2010 (Jan-Mar);1(1):e4 doi:10.5037/jomr.2010.1104 |
Early Effects of P-15 on Human Bone Marrow Stem Cells
1Orthopedic Clinic, University of Ferrara, Ferrara, Italy
2Department of Histology, Embryology and Applied Biology, University of Bologna, Bologna, Italy
3Department of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy
Corresponding author:
Department of Maxillofacial Surgery
University of Ferrara
Corso Giovecca 203
IT – 44100, Ferrara
Italy
Phone: 0039-0532-455582
Fax: 0039-0532-455582
E-mail: crc@unife.it
ABSTRACT
Objectives: Peptide-15 (P-15) is an analogue of the cell binding domain of collagen. P-15 has been shown to facilitate physiological to process in a way similar to collagen, to serve as anchorage for cells, and to promote the binding, migration and differentiation of cells. However, how P-15 alters osteoblast activity to promote bone formation is poorly understood. To study the osteoinductive properties of peptide P-15, we analyzed the expression levels of bone related genes in human mesenchymal stem cells treated with this biomaterial.
Material and Methods: Using real time Reverse Transcription-Polymerase Chain Reaction the quantitative expression of specific genes, like transcriptional factors (RUNX2 and SP7), bone related genes (SPP1, COL1A1, COL3A1, BGLAP, ALPL, and FOSL1) and mesenchymal stem cells marker (ENG) were examined.
Results: P-15 causes a considerable induction of osteoblast transcriptional factor like osterix (SP7) and of the bone related genes osteopontin (SPP1) and osteocalcin (BGLAP). In contrast the expression of endoglin (ENG) was markedly decreased in stem cells treated with P-15 respect to untreated cells, indicating the differentiation effect of this biomaterial on stem cells.
Conclusions: The present study shows the effect of P-15 on mesenchymal stem cells in
the early differentiation stages: P-15 is an inducer of osteogenesis on
human stem cells as indicated by the activation of bone related markers
SP7, SPP1 and BGLAP.
The results may allow a better understanding of the molecular mechanism
of bone regeneration and as a model for comparing other materials with
similar clinical effects.
J Oral Maxillofac Res 2010 (Jan-Mar);1(1):e4
doi:10.5037/jomr.2010.1104
Accepted for publication: 26 November 2009
Keywords: stem cells; biomaterial; P-15; differentiation; bone.
To cite this article: Early effects of P-15 on human bone marrow stem cells. J Oral Maxillofac Res 2010 (Jan-Mar);1(1):e4 |
Received: 30 October 2009 | Accepted: 26 November 2009 | Published: 1 April 2010
Copyright: © The Author(s). Published by JOMR under CC BY-NC-ND 3.0 licence, 2010.