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2011 Oct-Dec; Vol 2, No 4:e5 |
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Laminin Coating Promotes Calcium Phosphate Precipitation on Titanium Discs in vitro J Oral Maxillofac Res 2011 (Oct-Dec);2(4):e5 doi:10.5037/jomr.2011.2405 |
Laminin Coating Promotes Calcium Phosphate Precipitation on Titanium Discs in vitro
1Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden.
2Department of Prosthodontics, Faculty of Odontology, Sahlgrenska Academy, Göteborg University, Göteborg, Sweden. 3Promimic AB, Göteborg, Sweden. 4Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, Göteborg University, Göteborg, SwedenCorresponding author:
Department of Prosthodontics, Faculty of Odontology, Malmö University
205 06 Malmö
Sweden
Phone: +46 40 6658520
Fax: +46 40 6658503
E-mail: kostas.bougas@mah.se
ABSTRACT
Objectives: The objective of this study was to investigate the effect of a laminin coating on calcium phosphate precipitation on three potentially bioactive titanium surfaces in simulated body fluid.
Material and Methods: Blasted titanium discs were prepared by alkali and heat treatment (AH), anodic oxidation (AO) or hydroxyapatite coating (HA) and subsequently coated with laminin. A laminin coated blasted surface (B) served as a positive control while a blasted non coated (B-) served as a negative control. Surface morphology was examined by Scanning Electron Microscopy (SEM). The analysis of the precipitated calcium and phosphorous was performed by Energy Dispersive X-ray Spectroscopy (EDX).
Results: The thickness of the laminin coating was estimated at 26 Å by ellipsometry. Interferometry revealed that the coating process did not affect any of the tested topographical parameters on µm level when comparing B to B-. After 2 weeks of incubation in SBF, the alkali-heat treated discs displayed the highest calcium phosphate deposition and the B group showed higher levels of calcium phosphate than the B- group.
Conclusions: Our results suggest that laminin may have the potential to be used as a coating agent in order to enhance the osseoinductive performance of biomaterial surfaces, with the protein molecules possibly functioning as nucleation centres for apatite formation. Nevertheless, in vivo studies are required in order to clarify the longevity of the coating and its performance in the complex biological environment.
J Oral Maxillofac Res 2011 (Oct-Dec);2(4):e5
doi:10.5037/jomr.2011.2405
Accepted for publication: 16 September 2011
Keywords: laminin; titanium; biomaterials; calcium phosphates; dental implants; osseointegration.
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To cite this article: Laminin Coating Promotes Calcium Phosphate Precipitation on Titanium Discs in vitro. J Oral Maxillofac Res 2011;2(4):e5 URL: http://www.ejomr.org/JOMR/archives/2011/4/e5/v2n4e5ht.htm |
Received: 25 August 2011 | Accepted: 16 September 2011 | Published: 1 January 2012
Copyright: © The Author(s). Published by JOMR under CC BY-NC-ND 3.0 licence, 2012.