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2020 Jul-Sep; Vol 11, No 3:e4 |
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Evaluation of Collagen Membranes Coated with Testosterone and Alendronate to Improve Guided Bone Regeneration in Mandibular Bone Defects in Minipigs J Oral Maxillofac Res 2020;11(3):e4 doi:10.5037/jomr.2020.11304 Abstract | HTML | PDF | XML |
Evaluation of Collagen Membranes Coated with Testosterone and Alendronate to Improve Guided Bone Regeneration in Mandibular Bone Defects in Minipigs
1Department of Dentistry - Biomaterials, Radboudumc, Radboud University Nijmegen, Nijmegen, The Netherlands.
2Osteo-Pharma BV, Oss, The Netherlands.
Corresponding Author:
Department of Dentistry - Biomaterials
Radboud University Nijmegen
Dentistry 309, Radboudumc, Ph v Leijdenln 25
6525 EX NIJMEGEN
The Netherlands
Phone: +31 24 361 4027
E-mail: bart.vanoirschot@radboudumc.nl
ABSTRACT
Objectives: The purpose of the present in vivo study was to evaluate whether pericard collagen membranes coated with ancillary amounts of testosterone and alendronate in a poly-lactic glycolic acid (PLGA) carrier as compared to uncoated membranes will improve early bone regeneration.
Material and Methods: In each of 16 minipigs, four standardized mandibular intraosseous defects were made bilaterally. The defects were filled with Bio-Oss® granules and covered with a non-coated or coated membrane. Membranes were spray-coated with 4 layers of PLGA containing testosterone and alendronate resulting in 20, 50 or 125 μg/cm2 of testosterone and 20 µg/cm2 alendronate (F20, F50, F125). Non-coated membranes served as controls (F0). Animals were sacrificed at 6 and 12 weeks after treatment. Qualitative and quantitative histological evaluations of bone regeneration were performed. Differences between groups were assessed by paired Student’s t‐test.
Results: Light microscopical analysis showed new bone formation that was in close contact with the Bio-Oss® surface without an intervening non-mineralized tissue layer. Histomorphometric analysis of newly formed bone showed a significant 20% increase in area in the F125 coated membrane treated defects (40 [SD 10]%) compared to the F0 treated defects after 6 weeks (33 [SD 10]%, P = 0.013). At week 12, the total percentage of new bone was increased compared to week 6, but no increase in newly formed bone compared to F0 was observed.
Conclusions: The data from this in vivo study indicate that F125 collagen membranes coated with testosterone and alendronate resulted in superior bone formation (+24%) when normalized to control sites using uncoated membranes.
J Oral Maxillofac Res 2020;11(3):e4
doi: 10.5037/jomr.2020.11304
Accepted for publication: 27 September 2020
Keywords: bone regeneration; bone substitutes; mandible; surgery; membranes; minipig.
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To cite this article: Evaluation of Collagen Membranes Coated with Testosterone and Alendronate to Improve Guided Bone Regeneration in Mandibular Bone Defects in Minipigs J Oral Maxillofac Res 2020;11(3):e4 URL: http://www.ejomr.org/JOMR/archives/2020/3/e4/v11n3e4ht.htm |
Received: 23 July 2020 | Accepted: 27 September 2020 | Published: 30 September 2020
Copyright: © The Author(s). Published by JOMR under CC BY-NC-ND 3.0 licence, 2020.